Digital PCR (ddPCR) Services
Droplet Digital™ PCR is ideal for applications where high sensitivity and absolute quantification are desired.
Digital PCR can also detect minute gene expression fold changes, and find rare genetic events in mixed cell populations. Other benefits of Droplet Digital™ PCR include the ability to perform single-cell analysis, and the flexibility to analyze low abundance or challenging samples, such as cell-free DNA or environmental DNA.
Precision’s ddPCR capabilities and applications
Precision uses the Bio-Rad QX200™ ddPCR™ System for digital PCR assays – which in addition to high precision and sensitivity, has scalability to accommodate high throughput analyses. In addition, ddPCR can accept a range of sample types.
Precision has special expertise in utilizing ddPCR for gene therapy and cell therapy applications, both at the preclinical stage and for clinical sample analysis.
General Applications
- Rare sequence and mutation detection
- NGS pre- and post-run validation
- Sensitive copy number variation (CNV) detection
- Single cell analysis
- Gene expression and miRNA analysis
- Liquid biopsy: cfDNA
Cell Therapy Applications
- CAR-T biodistribution
- Cell therapy persistence / pharmacokinetics
- Vector copy number measurement (as an estimate of CAR vector delivery efficiency)
- Replication-competent lentivirus (RCL) assay
Gene Therapy Applications
- Viral vector biodistribution
- Viral shedding
- Oncolytic virus shedding
- Transgene expression
- Vector copy number estimation
- Gene editing event detection
Compatible sample types for ddPCR include low-abundance and challenging samples
- Tissue: Fresh, Frozen, FFPE
- Cells, exosomes
- Liquid biopsies: Blood, BM, CSF, Saliva, Tears, Urine, cfDNA, cfRNA
Oncology: Phase 1/2 dose escalation study in solid tumors
Study to determine expression level of 2 targeted genes – qPCR was requested. ddPCR recommended by Precision to deliver more sensitive and quantified data with less sample required.
Infectious disease: Cell-free HPV DNA detection
Liquid biopsy: detection of HPV infection by cell-free HPV DNA. ddPCR is ideal here for detection of low abundance cell-free DNA.
Gene therapy: Assess copy number in gene of interest in several tissue types
GLP study to determine vector copy number in 16 tissue types in NHP. Flexibility in sample requirements and high sensitivity in ddPCR are ideal for generating gene therapy biodistribution data.
Learn more about our genomics solutions:
Additional genomics services
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qPCR
Multiple platforms including the QuantStudio™ 12k Flex with OpenArray™ plates for rapid, multiplexed screening of dozens of genes and samples in a single run -
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NGS
Services including RNA-seq, whole exome sequencing, and targeted DNA/RNA sequencing utilizing both Illumina and Thermo-Fisher NGS platforms -
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NanoString
Panels that enable the simultaneous analysis of up to 800 genes; ideal for projects where highly multiplexed analysis is needed, such as oncology and autoimmune therapeutic development
